Bioimaging Core

Widefield Fluorescence Microscopes

Olympus BX61WI

  • Microscope Type: Fully motorized upright fluorescence microscope for fixed tissue and slide-based imaging

    • Fluorescence Illumination: High-intensity Xenon arc lamp for broad-spectrum excitation across UV–visible wavelengths

    • Filter Sets: Interchangeable fluorescence filter cubes covering common fluorophores (DAPI, CFP, GFP, YFP, TRITC, Cy5)

    • Objectives: Wide range of high–numerical aperture plan achromat and plan apochromat objectives (4X, 5X, 10X, 20X, 40X, 63X (oil), 100X (oil)

    • Motorization:

      • Motorized stage for precise XY positioning, tiling, and multi-position imaging

      • Motorized filter turret and focus drive for automated acquisition workflows

    • Imaging Capabilities:

      • Multichannel fluorescence imaging

      • Z-stack acquisition for 3D reconstruction

      • Large-area image tiling and stitching

    • Camera System: High-sensitivity sCMOS camera for low-noise detection and rapid acquisition

    • Software Integration: Supports automated multidimensional imaging (XYZ, time, channels) and quantitative analysis workflows

    • Applications: Ideal for immunofluorescence imaging of tissue sections (including skin), fluorescent reporter analysis, histopathology, and high-resolution documentation of labeled specimens

Olympus IX81

  • Microscope Type: Fully motorized inverted research microscope optimized for live-cell and in vitro imaging

  • Fluorescence Illumination: Metal halide fluorescence light source

  • Filter Sets: Interchangeable fluorescence filter cubes supporting common fluorophores (DAPI, CFP, FITC, RFP, TXRED, Cy5)

  • Objectives: Broad range of plan achromat and plan apochromat objectives (4x, 5x, 10x, 20X, 40X, 63x (oil)

  • Motorization:

    • Motorized XY stage for multi-position imaging, tiling, and automated scanning

    • Motorized Z-drive for precise focus control and z-stack acquisition

    • Motorized filter wheels/turrets for rapid channel switching

  • Imaging Capabilities:

    • Multichannel fluorescence imaging

    • Time-lapse imaging for dynamic cellular processes

    • Z-stacks for 3D reconstruction

    • Large-area image tiling and stitching

  • Camera System: High-sensitivity sCMOS camera for fast acquisition and low-light imaging

  • Environmental Compatibility: Fully compatible with stage-top incubation systems for temperature, CO₂, and humidity control

  • Software Integration: Supports multidimensional acquisition (XYZ, time, channels) and automated workflows

  • Applications: Ideal for live-cell imaging of keratinocytes, fibroblasts, and other skin-resident cells, as well as in vitro assays, migration studies, and fluorescence-based functional imaging