Bioimaging Core

Multiphoton Microscopes

Olympus FV1200MPE

  • Microscope Type: Upright multiphoton laser scanning microscope optimized for deep tissue and intravital imaging

  • Laser Source: Coherent Chameleon Vision II femtosecond pulsed laser, tunable ~680–1080 nm for excitation of a broad range of fluorophores

  • Scanning System: Galvanometric laser scanning with high spatial precision and flexible scan speeds

  • Detection: High-sensitivity non-descanned detectors (NDDs), with 2 Multialkali PMTs and 2 High Sensitivity GaAsP for efficient photon collection in scattering tissues

  • Optical Sectioning: Intrinsic multiphoton excitation enables subcellular resolution with reduced out-of-focus photobleaching

  • Imaging Depth: Capable of imaging hundreds of microns deep into intact tissues (e.g., skin, cornea, brain)

  • Objectives: Selection of 5, 10 and 20X dry objectives and 25X 105 NA water immersion objective optimized for live imaging

  • Motorization: Motorized stage and focus control for z-stacks, multi-position imaging, and longitudinal studies

  • Imaging Capabilities:

    • Multichannel fluorescence imaging

    • Time-lapse and longitudinal imaging in vivo

    • Z-stack acquisition for 3D reconstruction

  • Nonlinear Imaging: Supports label-free modalities such as second harmonic generation (SHG) for collagen and extracellular matrix visualization

  • Environmental Integration: Compatible with intravital imaging setups including anesthesia systems, heated stages, and tissue stabilization platforms

  • Applications: Ideal for intravital imaging of skin and ocular tissues, stem cell dynamics, immune cell behavior, vascular biology, and functional imaging (e.g., calcium signaling)

Evident FV5000MPE

  • Microscope Type: Next-generation upright multiphoton laser scanning microscope for deep tissue and intravital imaging

  • Laser System: Coherent Discovery NX providing two independently tunable beams (typically ~680–1300 nm), enabling simultaneous or sequential dual-wavelength excitation

  • Scanning System: Hybrid scanning architecture combining galvanometric and resonant scanners for flexible high-resolution and high-speed imaging

  • Detection: Advanced non-descanned detectors (high-sensitivity Hybrid) optimized for photon-efficient detection in highly scattering tissues

  • Optical Performance: Enhanced excitation efficiency and signal uniformity across depth, supporting improved imaging quality in thick specimens

  • Imaging Depth: Capable of imaging up to millimeter-scale depths depending on tissue type and optical properties

  • Objectives: Selection of 5, 10 and 20X dry objectives and 25X 105 NA water immersion objective optimized for live imaging

  • Motorization: Fully motorized stage and focus control for automated z-stacks, tiling, and multi-position longitudinal imaging

  • Imaging Capabilities:

    • Simultaneous multicolor imaging with reduced spectral crosstalk

    • High-speed time-lapse imaging (resonant scanning)

    • Deep z-stack acquisition for 3D reconstruction

  • Nonlinear Imaging: Supports SHG and THG for label-free visualization of extracellular matrix and structural components

  • Functional Imaging: Optimized for dynamic measurements such as calcium signaling and other genetically encoded reporters

  • Environmental Integration: Fully compatible with intravital imaging setups (anesthesia systems, temperature control, tissue stabilization platforms)

  • Applications: Ideal for advanced intravital imaging in skin and ocular tissues, neuronal activity mapping, immune cell dynamics, vascular biology, and longitudinal studies of tissue regeneration and disease